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  • James Kennard S. Jacob
  • Jennifer C. Paltiyan
  • Hezekiah Mirielle P. Aguinaldo
  • Ron Patrick Cuagdan Campos Isabela State University


Rain tree,, antioxidant,, cytotoxicity,, embryotoxicity,, Samanea saman,


Pacific rain tree (Samanea saman) of the Family Fabaceae was investigated for its pharmacological potentials. In this study, S. saman pods were tested for their cytotoxicity, embryotoxicity and antioxidant properties. The pods were collected and oven-dried prior to extraction with 95% ethanol for 48 hours and evaporation using rotary evaporator at 300 rpm. Using brine shrimp lethality assay, the extracts were found have cytotoxic effects against Artemia salina larvae with LC50 value of 4.65 ppm. Similarly, the ethanol extracts of rain tree pods exhibited embryotoxicity against zebra fish (Danio rerio). Zebra fish exposed to the rain tree pod extract for 48 hours were observed to have head and tail malformation, growth retardation, flexure, stunted tail, limited movement, coagulation, undetached tail, lack of somite and absence of heartbeat. The free radical scavenging activity of the samples were estimated using the stable 2, 2’diphenyl-1-1picrylhydrazyl (DPPH) radical assay. The extract recorded an EC50 of 70.92 ppm. Results of this study revealed that S. saman pods can be a potential source of antioxidants and other bioactive compounds with cytotoxic and teratogenic properties.


Ferdous, A., Mohammad, Z.I., Tajnin, A. (2010): Antioxidant, Antimicrobial and Cytotoxic Activities of Samanea saman (Jacq.) Merr. Standford Journal of Pharmaceutical Sciences (3): 11-17.

Lai, H.Y., Lim, Y.Y., Kim, K.H. (2010): Blechnum orientale Linn-a fern with potential as antioxidant, anticancer and antibacterial agent. BMC Complementary and Alternative Medicine 10(1): 15. doi: 10.1186/1472-6882-10-15.

Durr, P.A. (2001): The biology, ecology and agroforestry potential of the raintree, Samanea saman (Jacq.) Merr. Agroforestry systems 51(3): 223-237.

Vinodhini, S., Rajeswari, D.V. (2018): Review on Ethnomedical Uses, Pharmacological Activity and Phytochemical Constituents of Samanea saman(jacq.) Merr. Rain Tree. Pharmacognosy Journal 10(2): 202-209.

Kabir, M., Iqbal, Z.M., Shafiq, M. (2012): Traffic Density, Climatic Conditions and Seasonal Growth of Samanea Saman (Jacq.) Merr. On Different Polluted Roads of Karachi City, Pak. Journal of Botany 44(6): 1881-1890.

Iqbal Azhar, M., Mazhar, F., Ali, M. (2009): Some biological evaluations on Samanea saman. Pakistan Journal of Pharmacology 26(1): 47-53.

Rajkapoor, B., Anandan, R., Jayakar, B. (2002): Anti-ulcer effect of Nigella sativa Linn. against gastric ulcers in rats. Current Science 82(2): 177-179.

Jacob, J.K.S., David, E.S. (2016): Qualitative Phytochemical Analysis and Microbial Inhibitory Activities of Pacific Rain Tree (Samanea saman (Jacq.) Merr.) Pods. Advancements in Life Sciences 3(4): 125-129.

Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.J., McLaughlin, J.L. (1982): Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica 45(05): 31-34.

Schulte, C., Nagel, R. (1994): Testing acute toxicity in the embryo of zebrafish, Brachydanio rerio, as an alternative to the acute fish test: preliminary results. Atla 22(1): 12-19.

Dulay, R.M.R., Kalaw, S.P., Reyes, R.G., Cabrera, E.C. (2014): Embryo-toxic and teratogenic effects of Philippine strain of Lentinus tigrinus (tiger sawgill basidiomycetes) extract on zebrafish (Danio rerio) embryos. Annals of Biological Research 5(6): 9-14.

Chanda, S., Baravalia, Y. (2011): Brine shrimp cytotoxicity of Caesalpinia pulcherrima aerial parts, antimicrobial activity and characterization of isolated active fractions. Natural Product Research 25(20): 1955-1964.

Ukoha, P.O., Cemaluk, E.A., Nnamdi, O.L., Madus, E.P. (2011): Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry 5(8): 237-244.

Rath, S.C., Nayak, K.C., Pradhan, C., Mohanty, T.K., Sarkar, S., Mohanta, K.N., Paul, B.N., Giri, S.S. (2017): Evaluation of processed rain tree (Samanea saman) pod meal as a non-conventional ingredient in the diet of Catla catla Fry. Animal Nutrition and Feed Technology 17(2): 323-332.

Labieniec, M., Gabryelak, T. (2003): Effects of tannins on Chinese hamster cell line B14. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 539(1-2): 127-135.

Vinodhini, S. (2018): Review on ethnomedical uses, pharmacological activity and phytochemical constituents of Samanea saman (jacq.) Merr. rain tree. Pharmacognosy Journal 10(2): 202-209.

Green, B.T., Lee, S.T., Welch, K.D., Panter, K.E. (2013): Plant alkaloids that cause developmental defects through the disruption of cholinergic neurotransmission. Birth Defects Research Part C: Embryo Today: Reviews 99(4): 235-246.

Kirithika, T., Bhaigyabati, T., Gomathi, R., Usha, K. (2013): Preliminary Phytochemical Screening and Antioxidant Property of various Extracts of various Extracts of Albizia saman leaves. International Journal of Pharmaceutical Research in Bio-science 2(1): 315-323.

Arulipya, P., Lalitha, P., Hemalatha, S. (2010): In vitro antioxidant testing of the extracts of Samanea saman (Jacq.) Merr. Der Chemica Sinica 1(2): 73-79.

Sen, S., Chakraborty, R. (2011): Role of Antioxidants in Human Health. ACS Symposium Series 1083 Ch 1: 1–37.

Arora, D.S., Chandra, P. (2011): Antioxidant activity of Aspergillus fumigatus. ISRN Pharmacology 2011: 619395. doi: 10.5402/2011/619395.




How to Cite

Jacob, J. K. S., Paltiyan, J. C., Aguinaldo, H. M. P., & Campos, R. P. C. (2022). TOXICITY AND ANTIOXIDANT PROPERTIES OF PACIFIC RAIN TREE (Samanea saman (Jacq.) Merr.) PODS. Journal of Applied Biological Sciences, 16(1), 128–136. Retrieved from