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Ephedra alata, Phytochemical, antibacterial activity, antioxidant activity, methanolic/hydro-methanolic extract


Ephedra alata is a medicinal plant that has a long history of usage in folk medicine for the treatment of several diseases such as bronchial asthma, digestive system disorders and cancer. The present study aimed to elucidate the chemical constituents of the crude methanolic and hydro-methanolic extracts of Ephedra alata aerial part and to assess their antibacterial and antioxidant activities. In this study, the percentage yield of crude methanolic and hydro-methanolic extracts was 12.92% and 15.01% respectively. Phytochemical screening investigations using standard method showed that E. alata aerial part extracts was rich in flavonoids, reducing sugars, phenols and tannins and quantitative analysis using spectrophotometric assay revealed that the methanolic extract had the highest phenols and flavonoids contents (214.92 mg AGE/g extract and 30.74 mg CE/g extract, respectively). The results of the antibacterial activity by the agar well diffusion and microdilution assays showed that the both extracts displayed antibacterial effect, especially on gram positive strains with S. aureus being the most sensitive. Antioxidant activity was examined by the DPPH radical scavenging method. The methanolic extract has a significant inhibitory activity (IC50= 226.58 μg/mL). These findings revealed that E. alata extracts could be a natural source of antioxidants and antibacterials that can prevent several diseases.


Jaradat, N., Dacca, H., Hawash, M., Abualhasan, MN. (2021): Ephedra alata fruit extracts: phytochemical screening, anti-proliferative activity and inhibition of DPPH, α-amylase, α-glucosidase, and lipase enzymes. Journal of BMC Chemistry 15(1):41.

Dbeibia, A., Ben Taheur, F., Altammar, KA., Haddaji, N., Mahdhi, A., Amri, Z., Mzoughi, R., Jabeur, C. (2021): Control of Staphylococcus aureus methicillin resistant isolated from auricular infections using aqueous and methanolic extracts of Ephedra alata. Saudi Journal of Biological Sciences 29: 1021–1028. 10.1016/ j. sjbs.2021.09.071

Shahidi, F., Ambigaipalan, P. (2015): Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects: A review. Journal of Functional Foods 18: 820-897.

Ozcan, T., Akpinar-Bayizit, A., Yilmaz-Ersan, L., & Delikanli, B. (2014): Phenolics in human health. International Journal of Chemical Engineering and Applications 5(5): 393-396.

Ziani, B.E.C., Heleno, S.A., Bachari, K., Dias, M.I., Alves, M.J., Barros, L., Ferreira, I.C.F.R. (2019): Phenolic compounds characterization by LC-DAD- ESI/MSn and bioactive properties of Thymus algeriensis Boiss. &Reut. and Ephedra alata Decne. Food Research International116: 312-319. 08.0 41

Middleton, E., Kandaswami, C., Theoharides, T.C. (2000): The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease and cancer. Pharmacological Reviews 52: 673-839.

Ksouri, R., Megdiche, W., Debez, A., Falleh, H., Grignon, C., Abdelly, C. (2007): Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiology and Biochemistry 45(34): 244-249.

Ouguirti, N., Bahri, F., Bouyahyaoui, A., Wanner, J. (2021): Chemical characterization and bioactivities assessment of Artemisia herba-alba Asso essential oil from South-western Algeria. Natural Volatiles, Essential Oils 8(2): 27-36.

Al-Qarawi, A. A., Abd Allah, E. F., & Abeer, H. (2012): Effect of Ephedra alata on nucleic acids and nitrogen metabolism of seedborne Aspergillus flavus. Pakistan Journal of Botany 44(1): 425-428.

Jaradat, N., Hussen, F., & Al Ali, A. (2015): Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata. Journal of Materials Environmental Science 6(6): 1771-1778.

Ould el hadj, M.D., Hadj mahammed, M., Zabeirou, H. (2003): Place of the spontaneous plants samples in the traditional pharmacopoeia of the area of Ouargla (Septentrional east Sahara). Courrier du Savoir 3 : 47-51

Danciu, C., Muntean, D., Alexa, E., Farcas, C., Oprean, C., Zupko, I., Bor, A., Minda, D., Proks, M., Buda, V., Hancianu, M., Cioanca, O., Soica, C., Popescu, S., Dehelean, C.A. (2019): Phytochemical characterization and evaluation of the antimicrobial, antiproliferative and pro-apoptotic potential of Ephedra alata Decne. Hydroalcoholic extract against the MCF-7 breast cancer cell line. Molecules 24: 1-15. 10.3390/molecules24010013

Lamine, J.B., Boujbiha, M.A., Dahane, S., Cherifa, A.B., Khlifi, A., Chahdoura, H., Yakoubi, M.T., Ferchichi, S., El Ayeb, N., Achour, L. (2019): Α-amylase and aglucosidase inhibitor effects and pancreatic response to diabetes mellitus on wistar rats of ephedra alata areal part decoction with immunohistochemical analyses. Environ. Sci. Pollut. Res 26: 9739-9754. 10.1007/ s11 356-019-04339-3

Ibragic, S., Sofic, E. (2015): Chemical composition of various Ephedra species. Bosnian Journal of Basic Medical sciences15: 21-27.

Pandey, A., Tripathi, S. (2014): Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry JPP 115(25): 115-9.

Ozkok, A., D’arcy, B., Sorkun, K. (2010): Total phenolic acid and total flavonoid content of Turkish pine honeydew honey. Journal of ApiProduc and ApiMedical Science 2: 65 71.

Zou, Y., Lu, Y., Wei, D. (2004): Antioxidant activity of a flvonoid rich extract of Hypericum perforatum L. in Vitro. Journal of Agricultural and Food Chemistry52:5032-5039.

Ahmad, I., Beg, A.Z. (2001): Antimicrobial and Phytochemical Studies on 45 Indian Medicinal Plants against Multi-Drug and Resistant Human Pathogens. Journal of Ethnopharmacology 74: 113-123.

Aref, H. L., Bel Hadj Salah, K., Fekih, A., Chemli, R., Mars, M., Aouni, M., Chaumon, J.P., Said, K. (2011): Variability in antimicrobial activity of latex from two varieties of Ficus carica. African Journal of Microbiology Research 5(12): 1361-1367.

Thaiponga, K., Boonprakoba, U., Crosbyb, K., Cisneros-Zevallosc, L., Byrnec, D.H. (2006): Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis 19: 669-675. /j. jfca.2 006. 0 1.003

Zheng, CD., Duan, YQ., Gao, JM., Ruan, ZG. (2010): Screening for antilipase properties of 37 traditional Chinese medicinal herbs. Chin Med J 73(6): 319-324.

Akhtar, A., ul-Haq, I., Mirza., M. (2015): Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian Journal of Chemistry 11(8): 1223-1235. https://doi. org/ 10. 1016/j.arabjc.2015.01.013

Bourgou, S., Ezzine, Y., Ben Mansour, R., Dakhlaoui, S., Selmi, S., Bachkouel, S., Msaada, K., Aidi-Wannes, W., Hiroko, I., Megdiche-Ksouri, W. (2020): Preliminary phytochemical analysis, antioxidant, anti-inflammatory and anticancer activities of two Tunisian Ephedra species: Ephedra alata and Ephedra fragilis. South African Journal of Botany 135: 421-428. .10 16/j.sajb.2020.09.033

Jerbi, A., Zehri, S., Abdnnabi, R., Gharsallah, N., Kammoun, M. (2016): Essential oil composition, free-radical-scavenging and antibacterial effect from stems of Ephedra alata alenda in Tunisia. Journal of Essential Oil Bearing Plants 19: 1503-1509.

Al-Rimawi, F., Abu-Lafi, S., Abbadi, J., Alamarneh, A.A.A., Sawahreh, R.A., Odeh, I. (2017): Analysis of phenolic and flavonoids of wild Ephedra alata plant extracts by LC/PDA and LC/MS and their antioxidant activity. African Journal of Traditional, Complementary and Alternative Medicines 14: 130-141. ajtcam.v14i2.1

Mokrani, A., Madani, K. (2016): Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Sep. Purif. Technol. 162: 68-76. 1016/j. seppur.2016.01.043.




How to Cite

boussena, A. abdelhadi, Bahri, F., Bouyahyaoui, A., Kouidri, M., & Meziane, M. (2022). SCREENING OF PHYTOCHEMICAL, EVALUATION OF PHENOLIC CONTENT, ANTIBACTERIAL AND ANTIOXYDANT ACTIVITIES OF EPHEDRA ALATA FROM THE ALGERIAN SAHARA. Journal of Applied Biological Sciences, 16(2), 220–229. Retrieved from