Antimicrobial resistance, biofilm formation, and virulence gene profiles of foodborne bacterial isolates from animal-based foods
DOI:
https://doi.org/10.71336/jabs.1587Keywords:
S. aureus, Salmonella spp., antibiotic resistance, virulence, biofilmAbstract
This study aimed to investigate the antibiotic resistance profiles, biofilm-forming capacities, and virulence characteristics of bacteria isolated from 86 animal-based food products, including 25 raw milk, 25 cheese, and 36 raw chicken samples. A total of 40 isolates were identified, comprising 14 Macrococcus caseolyticus, 10 Staphylococcus aureus, 9 Hafnia alvei, and 7 Salmonella spp. isolates. The results demonstrated that slime production was detected in all isolates, while moderate to strong biofilm formation was observed in 90% of the isolates. Among Gram-negative bacteria, resistance to ampicillin was detected in 85.7% of Salmonella spp. and 45.5% of H. Alvei isolates. However, all Gram-negative isolates were susceptible to chloramphenicol, ciprofloxacin, and streptomycin (100%). Regarding Gram-positive bacteria, S. aureus isolates exhibited 20% resistance to tetracycline and erythromycin, whereas M. caseolyticus isolates showed notably higher erythromycin resistance (57.1%). In contrast, all Gram-positive isolates were susceptible to gentamicin and chloramphenicol (100%). Virulence gene analysis revealed that among the S. aureus isolates tested, 30% were positive for icaA and 70% carried These posts must be removed.. Furthermore, among the seven Salmonella spp. isolates analyzed, 85.7% were positive for the mgtC gene, while all isolates (100%) harbored the sopB gene. In conclusion, the detection of S. aureus and Salmonella spp. in animal-based food products, particularly isolates exhibiting strong slime production, biofilmforming ability, and antibiotic resistance, represents a significant public health concern. These findings emphasize the critical importance of maintaining strict hygiene and sanitation practices throughout the production, processing, storage, and distribution stages under appropriate cold chain conditions.
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