Molecular Genetic Analysis of Three Turkish Local Silkworm Breeds (Bursa Beyazı, Alaca and Hatay Sarısı) by RAPD-PCR Method
Keywords:
Bombyx mori, silkworm, RAPD-PCR, genetic polymorphism.Abstract
In this study PCR-based Randomly Amplified Polymorphic DNA (RAPD) method was used to determine the genetic
variation in three local silkworm breeds (Bursa Beyazı, Alaca and Hatay Sarısı) of Turkey. These three breeds were
analysed with 23 RAPD primers. 34 polymorphic loci were observed with 5 primers of them. Percentage of polymorphic
loci were varied between 17.65 and 55.88. Nei’s genetic distance was 0.0822 between Hatay Sarısı-Alaca and 0.1106
between Bursa Beyazı -Alaca. The diversity within the populations (Hs) was found 0.1334 and the magnitude of
differentiation among the populations (GST) was found 0.2968.