ANTHELMINTIC ACTIVITIES OF STEM BARK OF PARKIA BIGLOBOSA ON WEST AFRICAN DWARF GOATS INFECTED WITH HAEMONCHUS CONTORTUSAbstract views: 74 / PDF downloads: 216
Keywords:Egg counts, , extract,, faecal,, goats, , phytochemicals
The anthelmintic effects of stem bark extracts of Parkia biglobosa was evaluated on West African Dwarf (WAD) goats. The stem bark of the plant was obtained from Kwara state of Nigeria. The extraction was done and yielded Crude Methanol Stem Bark Extracts (CMSBE), Ethyl acetate (EA) fraction and Aqueous (AQ) fraction. The anthelmintic potentials were studied in vivo in 18 WAD goats in six completely randomised groups, A, B, C, D, E and F with three animals per group. Group A was treated with 5mL/kg of distilled water as negative control, group B with 6.25mg/kg of Albendazole (ABZ)(positive control), groups C and D with 1000mg/kg and 2000mg/kg of CMSBE respectively and groups E and F with 1000mg/kg of EA fraction and AQ fraction each respectively. Faecal samples were collected for two weeks after treatment to evaluate faecal egg counts. After 16th day, one animal was euthanized from each group to determined percentage deparasitization. The result from this study revealed that, the phytochemical constituents present in CMSBE were alkaloid, anthraquinones, cardiac glycosides, glycosides, flavonoids, oils, phlobatannins, reducing sugar, saponins, sterols/steroids, tannin (condensed and hydrolysable) and terpenoids. In vivo result showed significant (P<0.05) reduction in egg per gram (EPG) faeces in group B, C, D and E when compared to group A, all at 16th day post-treatment. Group F showed less efficacy in EPG reduction. The deparasitisation obtained in groups D and E were higher than group B, though without statistical significant difference. The study has shown that, CMSBE and EA fraction of P. biglobosa exhibited in vivo anthelmintic activities at 2000mg/kg and 1000mg/kg respectively that are sufficiently comparable to ABZ, hence, have potentials as a novel anthelmintic ethnobotanic preparation for control of H. contortus in WAD goats. It is therefore recommended to carry out a further research on larger population size of goats.
Sahlu, T., Dawson, L.J., Gipson, T.A., Hart, S.P., Merkel, R.C. (2009): ASAS Centennial Paper: Impact of animal science research on United States goat production and predictions for the future, Journal of Animal Science 87: 400-418.
Tariq, K.A., Chishti, M.Z., Ahmad, F. (2010): Gastro-intestinal nematode infections in goats relative to season, host, sex and age from the Kashmir valley. Indian Journal of Helminthology 84: 93– 97.
Owhoeli, O., Elele, K., Gboeloh, L.B. (2014): Prevalence of Gastrointestinal Helminths in Exotic and Indigenous Goats Slaughtered in Selected Abattoirs in Port Harcourt, South-South, Nigeria. Chinese Journal of Biology 435913: 8pp.
Maciel, M.V., Morais, S.M., Bevilaqua, C.M.L., Camurca-Vasconcelos, A.L.F., Costa, C.T. C., Castro, C.M.S. (2006): Ovicidal and larvicidal activity of Meliaazedarach extracts on Haemonchus contortus. Veterinary Parasitology 140:98-104.
Athanasiadou, S., Houdijk, J., Kyriazakis, I. (2008): Exploiting synergisms and interactions in the nutritional approaches to parasite control in sheep production systems. Small Ruminant Research 76 (1-2): 2-11.
Sawleha, Q., Dixit, A.K., Pooja, D. (2010): Use of medicinal plants to control Haemonchus contortus infection in small ruminants. Veterinary World 3(11):515-518.
Waller, P.J. (2006): Sustainable nematode parasite control strategies for ruminant livestock by grazing management and biological control. Animal Feed Science and Technology 126: 277-279.
Soetan, K.O., Aiyelaagbe, O.O. (2009): The Need for Bioactivity-Safety Evaluation and Conservation of Medicinal Plants- A Review. Available online at http://www.academicjournals.org/JMPR. Journal of Medical Plants Research 3(5): 324-328.
Sandoval-Castro, C.A., Torres-Acosta, J.F.J., Hosteb, H., Salemd, A.Z.M., Chan-Péreza, J.I. (2012): Using plant bioactive materials to control gastrointestinal tract helminths in livestock. Journal of Animal Feed Science and Technology 176: 192-201.
Athanasiadou, S., Githiori, J., Kyriazakis, I. (2007): Medicinal plants for helminth parasitecontrol: Facts and fiction. Animal Consortium 1: 1392-1400.
Udobi, C.E., Onaolapo, J.A. (2009): Phytochemical analysis and antibacterial evaluation of leaf, stem bark and root of the African locust bean (Parkia biglobosa). Journal of Medical Plants Resources 3: 338-344.
Tokoudagba, J.M., Augera, C., Bréant, L., N'Gom, S., Chabert, P., Idris-Khodja, N., Schini-Kerth, V.B. (2010): Procyanidin-rich fractions from Parkia biglobosa (Mimosaceae) leaves cause redox-sensitive endothelium-dependent relaxation involving NO and EDHF in porcine coronary artery. Journal of Ethnopharmacology 132: 246–250.
Etuk, E.U., Bello, S.O., Isezuo, S.A., Mohammed, B.J. (2010): Ethonobotanical survey of medicinal plants used for the treatment of diabetes mellitus in the north western region of Nigeria. Asian Journal of Experimental Biology Science 2(1):55-59.
Gronhaug, T.E., Glaeserud, S., Skogsrud, M., Ballo, N., Bah, S., Diallo, D., Paulsen, B.S. (2008): Ethnopharmacological survey of six medicinal plants from Mali. West African Journal of Ethnopharmacology 4:4-26.
Tijani, A.Y., Okhale, S.E., Salawu, T.A., Onigbanjo, H.O., Obianodo, L.A., Akingbasote, J.A., Emeje, M. (2009): Anti diarrheal and antibacterial properties of crude aqueous stem bark extract and fractions of P. biglobosa (Jacq) R.Br Ex G. Don. African Journal of Pharmacy and Pharmacology 7:347-353.
Soetan, K.O., Lasisi, O.T., Agboluaje, A.K. (2011): Comparative assessment of in-vitro anthelmintic effects of the aqueous extracts of the seeds and leaves of the African locust bean (Parkia biglobosa) on bovine nematode eggs. Journal of Cell and Animal Biology 5 (6):109-112.
Builders, M.I., Isichie, C.O., Aguiyi, J.C. (2012): Toxicity Studies of the Extracts of Parkia biglobosa Stem Bark in Rats. British Journal of Pharmaceutical Research 2(1): 1-16.
Emmanuel, O.A., David, A.A., Olayinka, A.A., Mobolaji, F.A., Matthew, O.O., Anthony, I.O. (2013): Preliminary Phytochemical Screening and Antibacterial Properties of Crude Stem Bark Extracts and Fractions of Parkia biglobosa (Jacq.) Molecules 18 (7): 8485-8499.
Lorke, D. (1983): A new approach to practical acute toxicity testing. Arch. Toxicology 54:275-287.
Ibrahim, M.A. (1984): Evaluation of the activities of some West African Traditional anthelmintic herbs against Nippostrongylus brasiliensis in rats. M.Sc. Thesis, Department of Veterinary Physiology and Pharmacology, Ahmadu Bello University, Zaria pp. 116.
Loomis, T.A. (1978): Essentials of Toxicology. Third edition. Lea and Fibiger, Philadephia pp 198.
Kimani, D., Kareru, P.G., Njonge, F.K., Kutima, H.L., Nyagah, G.C., Rechab, S.O., Wamburu, R.W., Karanja, J.M. (2014): Control of gastro-intestinal nematodes in ruminants using plant extracts. Scientific conference proceeding pp.193-198.
Evans, W.C. (2002): Trease and Evans’ Pharmacology 15th Edition, W.B. Saunders, New York pp. 221-393.
Edeoga, H.O., Okwu, D.E., Baebie, B.O. (2005): Phytochemical constituents of some Nigerian medicinal plants: African Journal of Biotechnology 4 (7):685-688.
Makun, H.J., Ajanusi, O.J., Lakpini, C.A.M., Ehoche, O.W., Rekwot, P. I. (2008): Response of Red Sokoto and Sahelian Goats to Trickle Haemonchus contortus Infection. Journal of Biological Sciences 8: 753-759.
Soulsby, E.J.L. (1986): Helminths, Arthropods and protozoa of domesticated Animals, 7th Edition Bailliere Tindall, London pp. 119-218.
Hansen, J., Perry, B. (1994): The Epidemiology, Diagnosis and control of Helminth parasites of ruminants. International Livestock Center for Africa, Addis Ababa Ethiopis pp: 90-100.
Anaeto, M., Tayo, G.O., Chioma, G.O., Afolabi, A.A. (2009): Comparative study of Albendazole and Papaya seed in the control of Gastrointestinal Nematodes in Goats. Journal of life and physical sciences, acta SATECH 3(1): 25-28.
Chiejina, S.N., Behnke, J.M., Musongong, G.A., Nnadi, P.A, Ngongeh, L.A. (2010): Resistance and resilience of West African Dwarf goats of the Nigerian savanna zone exposed to experimental escalating primary and challenge infections with Haemonchus contortus. Journal of Veterinary Parasitology 171: 81–90.
Lucas, A.N., Amaechi, O. (2015): Comparative Response of the West African Dwarf Goats to Experimental Infections with Red Sokoto and West African Dwarf Goat Isolates of Haemonchus contortus. Journal of Pathogens 728210: 6.
Worku, M., Franco, R., Miller, J. H. (2009): Evaluation of the Activity of Plant Extracts in Boer Goats. American Journal of Animal and Veterinary Sciences 4 (4): 72-79.
Sujon, M.A., Mostofa, M., Jahan, M.S., Das, A.R., Rob, S. (2008): Studies On Medicinal Plants Against Gastroinstestinal Nematodes Of Goats. Bangladesh Journal of Veterinary Medicine 6 (2): 179–183.
Arundel, J.H. (1985): Veterinary anthelmintics. University of Sydney Postgraduate Foundation in Veterinary Science, New South Wales, Australia. Journal of Veterinary, Revised 26: 118.
MAFF (Ministry of Agriculture, Fisheries and Food). (1986): Manual of Veterinary Parasitological Laboratory Techniques. Her Majesty’s Stationary Office, London.
Ezekwe, C.I., Anaya, C.A., Okechukwu, P.C.U. (2013): Effects of Methanol Extract of Parkia biglobosa Stem Bark on the Liver and Kidney Functions of Albino Rats. Global Journal of Biotechnology and Biochemistry 8 (2):40-50.
Millogo-Kone, H., Guissou, I.P., Nacoulma, O., Traore, A.S. (2006): Study of the antibacterial activity of the stem bark and leaf extract of Parkia biglobosa (Jacq) Benth on Staphylococcus aureus. African Journal of Traditional Complentary and Alternative Medicine 3(2): 74-78.
Banwo, G O., Abdullahi, I., Duguryil, M. (2004): The antimicrobial activity of the stem bark of Parkia clappertoniana keay family Leguminosae against selected microorganisms. Nigerian Journal of Pharmarceutical Research 1: 16-22.
Adeyemi, O.O., Okpo, S.O., Young-Nwafor, C.C. (2009): The relaxant activity of the methanolic extract of Acanthus montanus on intestinal smooth muscles. Journal of Ethnopharmacology 68(1-3): 169-173.
Ciulei, I. (1982): Practical Manuals on the Industrial Utilization of Chemical and Aromatic Plants.Methodology for Analysis of Vegetable Drugs.1st edition, Ministry of Chemical Industry, Bucharest pp. 67.
Bliss, D.H., Moore, R.D., Kvasnicka, W.G. (2008): Parasite resistance in U.S. cattle. American Association of Bovine Practitioners 41: 109-114.
Coles, G.C., Bauer, F.H.M., Borgsteede, S., Greerts, S., Klei, T.R., Taylor, M.A., Waller, P.J. (1992): World association for the advancement of veterinary parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 44: 35-44.
Naandam, J., Iddrisu, R. (2010): Effect of Parkia biglobosa (Dawadawa) Pod extracts on strongyle ova in sheep. Animal Research International 7(3):1267–1273.
Birkett, D.J. (1995): Pharmacokinetics made easy: 10 Pharmacodynamics-the concentration-effect relationship. Australian Prescription 18:102-4.
Githiori, J.B. (2004): Evaluation of anthelmintic properties of ethnoveterinary plant preparations used as livestock dewormers by Pastoralists and small holder farmers in Kenya. Doctoral thesis ISSN 1401-6257.
Wabo, P.J., Tankoua, O.F., Yondo, J., Komtangi, M.C., Mbida, M., Bilong Bilong, C.F. (2011): The in vitro effects of aqueous and ethanolic extract of leaves of Ageratum conizoides (Asteraceae) on three life cycle stages of the parasitic Nematode Heligmosomoides bakeri (Nematoda: Heligmosomatidae). Journal of Veterinary Medicine International 140293: 1-5.
Sina, S., Traoré, S.A. Parkia biglobosa (Jacq.) R.Br.exG.Don. In:
Oyen, I.P.A, Lemmens R.H.M.J. (2002): (Editors), PROTA (Plant Resources of Tropical Africa / Ressources végétales de l’Afrique tropicale), Wageningen, The Netherlands 1-200.
Max R.A., Buttery, P.J., Wakelin, D., Kimambo, A.E., Kassuku, A.A., Mtenga, I.A. (2003): The potential of controlling gastrointestinal parasitic infections in tropical small ruminants using plants high in tannins or extracts from them. In: Proceedings of the Third DFID Livestock Production Programme Link Project (R7798) Workshop for Small Ruminant Keepers. Izaak Walton Inn, Embu, Kenya, 4 – 7 February 2003.
Octhere, G.V., Naandam, J. (2015): Effect of pounded dawadawa (Parkia biglobosa) pod husk extract on strongyle in west african dwarf (WAD) goats. UDS International Journal of Development [UDSIJD] 1 (1): 11-16.
Min, B.R., Hart, S.P. (2003): Tannins for suppression of internal parasites. Langston University, Langston. Pub. American society of animal science. Journal of Animal Science 81(2): 102-109.
Max, R.A., Wakelin, D., Dawson, J., Kimambo, A. E., Mtenga, L.A., Buttery, P.J. (2007). Effects of tanniniferous browse meal on nematode faecal egg counts and internal parasite burdens in sheep and goats. University of Nottingham. Journal of Animal Science 37(2): 97-104.
Cheng, H.Y., Lin, C.C., Lin, T.C. (2002): Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn. Antiviral Resource 55: 447–455.
Perumal, S.R., Ignacimuthu, S., Sen, A. (1998): Screening of 34 Indian
medicinal plants for antibacterial properties. Journal Ethanopharmacology 62: 173–182.
Paolini, V., Bergeaud, J.P., Grisez, C., Prevot, F., Dorchies, P., Hoste, H. (2003): Effects of condensed tannins on goats experimentally infected with Haemonchus contortus. Veterinary Parasitology 113(3-4): 253-261.
Paolini, V., De La Farge, F., Prevot, F., Dorchies P.H., Hoste, H. (2005): Effects of the repeated distribution of sainfoin hay on the resistance and the resilience of goats naturally infected with gastrointestinal nematodes. Veterinary Parasitology 127: 277–283.
Ademola, I.O., Fegbemi, B.O., Idowu, S.O. (2005): Anthelmintic activity of Extracts of Spondias mombin against gastrointestinal nematodes of sheep: Studies in vitro and vivo. Tropical Animal Health Production 37:223– 235.
Harder A. (2002): Chemotherapeutic approaches to nematodes: current knowledge and outlook. Parasitology Research 88: 272-277.
Susan, S. (2016): Understanding anthelmintics. Maryland Small Ruminant 1-14.
Williams, S., Botros, S., Ismail, M., Farghally, A., Day, T.A., Bennett, J.L. (2001): Praziquantel-induced tegumental damage in vitro is diminished in schistosomes derived from Praziquantel-resistant infection. Parasitology 122: 63-66.
Xiao, S.H., Guo, J., Chollet, J., Wu, J.T., Tarma, M., Utizinger, J. (2004): Effect of artemether on Schistosoma mansoni: dose-efficacy relationship, and change in worm morphology and histopatholoy. Chineese Journal of Parasitological Diseases 22: 148-153.
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