Antimicrobial Activity of Various Extracts of Satureja hortensis L.
Keywords:
Antimicrobial activity, disc diffusion method, SEM, spices, Satureja hortensisAbstract
The antimicrobial activities of ethyl acetate and methanol extracts of Satureja hortensis L. were studied. These extracts
were tested in vitro against 10 bacterial species, 4 yeasts species and strains by the disc diffusion method. The results
indicated that the methanol extract of S. hortensis is more effective against tested microorganisms than ethyl acetate. While
ethyl acetate extract showed inhibition effect against Pseudomonas aeruginosa (ATCC 27853) and Bacillus subtilis (RSHI),
methanol extract affected Bacillus subtilis (RSHI), Escherichia coli (ATCC 25922), Shigella (RSHI), Staphylococcus aureus
(ATCC 29213) and Pseudomonas aeruginosa (ATCC 27853). The cells of microorganisms treated with plant extracts and
normal microorganism cells were observed by scanning electron microscope. It was apparent that cells after treated with S.
hortensis are damaged.