SCREENING OF FRESHWATER MICROALGAE SPECIES FOR OCCURRENCE OF LECTINS AND THEIR CARBOHYDRATE-BINDING SPECIFICITYAbstract views: 136 / PDF downloads: 269
Keywords:Microalgae, Lectins, Hemagglutination activity, Carbohydrate-binding specificity
Lectins are a group of proteins with specific carbohydrate-binding properties, gained importance as a potential bio-component for applications in therapeutic and biotechnology field. They are naturally present in various sources such as plants, algae, animals, bacteria, fungi, and viruses. Detection of multiple disease biomarkers which are present in the biological samples can be analyzed by using these proteins as a diagnostic tool. In the present study, forty different freshwater microalgae were isolated from various local water-bodies and characterized as Chlorophyta and Ochrophyta strains. Hemagglutination activity of algal extracts was conducted for all isolated strains to evaluate the presence of lectin content using both native and enzyme-treated erythrocytes of different animals such as sheep, goat, chicken, and humans. Further, the hemagglutination inhibition test was carried out by using various carbohydrates and glycoproteins. All the tested algal extracts exhibited hemagglutination activity for at least one source of erythrocytes. With sheep erythrocytes 60% of the total algae tested showed agglutination, 45% with chicken erythrocytes, 20% with goat erythrocytes, and 5% with human O-type erythrocytes. Among 40 isolates, 24 species exhibited higher hemagglutinating titer value with trypsin treated erythrocytes. Whereas, specific oligosaccharides and glycoproteins inhibited the hemagglutination activity of the algal extracts. Hence, obtained results suggest that native freshwater microalgae can be a good source of lectins with biological significance.
Cardoso, K. H. M., Guaratini, T., Barros, M. P., Falcao, V. R., Tonon, A. P., Lopes, N. P., Campos, S., Torres, M. A., Souza, A. O., Colepicolo, P., Pinto, E. (2007): Metabolities from algae with economical impact. Comparative Biochemical Physiology 146: 66-78.
Lam, S. K., Ng, T. B. (2011): Lectins: production and practical applications. Applied Microbiology Biotechnology 89: 45–55.
Swamy, M. L. A. (2012): Marine algal sources for treating bacterial diseases. Advance in Food Nutrition and Research 64: 71–84.
Nascimento, K. S., Cunha, A. I., Nascimento, K. S. (2011): An overview of lectins purification stratergies. Journal of Molecular Recognition 25: 527-541.
Teixeira, E. H., Arruda, F. V. S., Nascimento, K. S. (2012): Biological applications of plants and algae lectins: An overview. In: Chang CF, ed. Carbohydrates-comprehensive studies on glycobiology and glycotechnology. Croatia: In Tech: 533–558.
Sharon, N., Lis, H. (2003): Lectins, Second edn. Kluwer, Dordrecht.
Kawakubo, A., Makino, H., Ohnishi, J., Hirohara, H., Hori, K. (1997): The marine red alga Eucheuma serra J. Agardh, a high yielding source of two isolectins. Journal of Applied Phycology 9: 331–338.
Pinto, V. P. T., Debray, H., Dus, D., Teixeira, E. H., Oliveira, T. M., Carneiro, V. A., Teixeira, A. H., Filho, G. C., Nagano, C. S., Nascimento, K. S., Sampaio, A. H., Cavada, B. H. (2009): Lectins from the red marine algal species Bryothamnion seaforthii and Bryothamnion triquetrum as tools to differentiate human colon carcinoma cells. Advance Pharmacology of Science 4: 1-10.
Singh, R. S., Thakur, S. R., Bansal, P. (2013): Algal lectins as promising biomolecules for biomedical research. Critical Reviews in Microbiology Early Online: 1–12.
Singh, R. S., Tiwary, A. K., Kennedy, J. F. (1999): Lectins: Sources, activities and applications. Critical Reviews in Biotechnology 19: 145–178.
Nascimento, K. S., Nagano, C. S., Nunes, E. V., Rodrigues, G. V., Calvete, J. J., Saker, S., Sampaio, W. R. L., Farias, S. A. H. (2006): Isolation and characterization of a new agglutinin from the red marine alga Hypnea cervicornis. J Agardh Biochem Cell Biol 84: 49–54.
Coelho, L. C. B. B., Silva, P. M. S., Lima, V. L. M., Pontual, E. V., Paiva, P. M. G., Napoleao, T. H., Correia, M. T. S. (2017): Lectins, interconnecting proteins with biotechnological/pharmacological and therapeutic applications. Evidence Based Complement Alternative Medicine 1594074: 1–22.
Franz, H. (1988): The ricin story. Advance Lectin Research 1: 10-25.
Boyd, W. C., Almodovar, L. R., Boyd, L. G. (1966): Agglutinins in marine algae for human erythrocytes. Transfusion 6: 82–83.
Rogers, D. J., Hori, K. (1993): Marine algal lectins: new developments. Hydrobiologia 260/261: 589–593.
de Oliveira, A. S., Lossio, C. F., Rangel, A. J., Martins M, G, Q., do Nascimento, F. E. P., de Andrade M. L. L., Cavada, B. S., Lacerda, S.R., do Nascimento, K. S. (2017): Detection, purification and characterization of a lectin from freshwater green algae spirogyra spp. An Academic Bras Cienc 89: 2113-2117.
Awoyinka, O. A., Dada, O. O. (2011): Partial Purification and Characterization of Lectin from the Seeds of Cissus poplunea. European Journal of Medicinal Plants 1: 130-139.
Ganapati, G. B., Kartika, N. S., Nagaraja, N. N., Vivek, V. N., Lingaraju, S., Ramesh, S. B., Shashikala, R. I., Suguna, K., Bale, M. S. (2010): Purification, characterization and molecular cloning of a monocot mannose-binding lectin from Remusatia vivipara with nematicidal activity. Glycoconjugates Journal 27: 309–320.
Praseptiangga, D. (2015): Algal lectins and their potential uses. Squalen Bull Mar Fish Post harvest Biotech 10: 89-98.
Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. (2004): Haemagglutinating and antibiotic activities of freshwater microalga. World Journal of Microbiology and Biotechnology 20: 817–825.
Hung, L. D., Ly, B. M., Trang, V. T. D., Ngoc, N. T. D., Hoa, L. T., Trinh, P. T. H. (2012): A new screening for hemagglutinins from Vietnamese marine macroalgae. Journal of Applied Phycology 24: 227–235.
Hori, K., Oiwa, C., Miyazawa, K., Ito, K. (1988): Evidence for wide distribution of agglutinins in marine algae. Botany Marine 31:133–138.
Chiles, T. C., Bird, K. T. (1989): A comparative study of the animal erythrocyte agglutinins from marine algae. Comprehensive Biochemical Physiology 94B: 107–111.
Hori, K., Miyazawa, K., Ito, K. (1990): Some common properties of lectins from marine algae. Hydrobiologia 204/205: 561–566.
Dalton, S. R., Longley, R. E., Bird, K. T. (1995): Hemagglutinins and immunomitogens from marine algae. Journal of Marine Biotechnology 2: 149-155.
Liao, W. R., Huang, R. (2000): Agglutination of human and animal erythrocytes in marine unicellular algae. Journal of Indian Microbiology and Biotechnology 24: 262–266.
Silva, A. J., Cavalcanti, V. L. R., Porto, A. L. F., Gama, W. A., Brandao-Costa, R. M. P., Bezerra, R. P. (2019): The green microalgae Tetradesmus obliquus (Scenedesmus acutus) as lectin source in the recognition of ABO blood type: purification and characterization. Journal of Applied Phycology 4: 1-10.
Hori, K., Miyazawa, K., Ito, K. (1986): Preliminary characterization of agglutinins from seven marine algal species. Bulletin in Japanese Society of Science Fish 52: 323–331.
De Souza, B. W. S., Teixeira, D. I. A., Andrade, F. K., Melo, M. R. S., Munoz, A. M., Freitas, A. L. P. (2007): A Survey of Antarctic Algae for agglutinins. Oecol Bras 11: 122-130.
Bird, K. T., Chiles, T. C., Longley, R. E., Kendrick, A. F., Kinkema, M. D. (1993): Agglutinins from marine macroalgae of the Southeastern United States. Journal of Applied Phycology 5: 212–213.
Alvarez-Hernandez, S., De Lara-Isassi, G., Arreguin-Espinoza, R., Arreguin, B., Hernandez-Santoyo, A., Rodriguez-Romero, A. (1999): Isolation and partial characterization of giraffine, a lectin from the Mexican endemic alga Codium giraffa Silva. Botany Marine 42: 573–580.
Freitas, A. L. P., Teixeira, D. I. A., Costa, F. H. F., Farias, W. R. L., Lobato, A. S. C., Sampaio, A. H., Benevides, N. M. B. (1997): A new survey of Brazilian marine algae for agglutinins. Journal of Applied Phycology 9: 495-501.
Ambrosio, A., Sanz, L., Sanchez, E., Wolfenstein-Todel, C., Calvete, J. (2003): Isolation of two novel mannanand L-fucose-binding lectins from the green alga Enteromorpha prolifera: biochemical characterization of EPL-2. Archieve Biochemistry Biophysics 415: 245–250.
Wang, S., Zhong, F. D., Zhang, Y. J., Wu, Z. J., Lin, Q. Y., Xie, L. H. (2004): Molecular characterization of a new lectin from the marine alga Ulva pertusa. Acta Biochim Biophysics Sin 36: 111–117.
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