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  • Hamza Belkhodja Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Department of Biology, University of Mustapha Stambouli, Mascara, ALGERIA.
  • Boumediene Meddah
  • Khadidja Sidelarbi
  • Djilali Bouhadi
  • Bouchra Medjadel
  • Amel Brakna


Diclofenac,, essential oil,, Eucalyptus globulus,, extraction,, inflammation,


This work was conducted as part of evaluation of the in vitro and in vivo anti-inflammatory activities of the essential oil extracted from a plant that belongs to the family of Myrtaceae: Eucalyptus globulus. The E. globulus essential oil was extracted by hydro-distillation. First, the in vitro anti-inflammatory activity of E. globulus essential oil was evaluated using the protein denaturation inhibition method compared to the reference drug Diclofenac. Then, the anti-inflammatory effect of E. globulus essential oil was studied in vivo using the 1% dextran edema model in the Wistar rat. The extraction of the essential oil gave a yield of 0,41 ± 0,01%. From the results obtained, it was noted that the E. globulus essential oil (250 μg/ml) and the drug Diclofenac (250 μg/ml) are capable of inhibiting denaturation of proteins with very high percentages. Thus, the assessment of the in vivo anti-inflammatory effect indicated that the treatment with the E. globulus essential oil showed a reduction of inflammatory reaction with different degrees of inhibition. The excellent anti-inflammatory potential of the essential oil of E. globulus may provide supports in the treatment of pathologies such as painful and inflammatory disorder. This shows that the essential oil of E. globulus would exert several beneficial effects by virtue of its phytochemicals and pharmacological potentials and could be harnessed in drug formulation.


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How to Cite

Belkhodja, H., Meddah, B., Sidelarbi, K., Bouhadi, D., Medjadel, B., & Brakna, A. (2022). IN VITRO AND IN VIVO ANTI-INFLAMMATORY POTENTIAL OF EUCALYPTUS GLOBULUS ESSENTIAL OIL. Journal of Applied Biological Sciences, 16(1), 80–88. Retrieved from